Analysis of HGPRT? CRM+ human lymphoblast mutants
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چکیده
منابع مشابه
Analysis of HGPRT- CRM+ human lymphoblast mutants.
Three 6-thioguanine-resistant mutants of the human diploid lymphoblast line MGL-8 were studied. The inactivation by heat of both HGPRT activity and antigenicity of the HGPRT immunologically cross-reacting material of the A30 mutant cells were not protected by PRPP, indicating that the HGPRT in A30 cells has an altered PRPP binding site, leading to lack of stabilization and rapid degradation of ...
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From a mass culture of diploid human lymphoblasts we have isolated a subline resistant to 1 muM ouabain, and from this, a subline resistant to 10 muM ouabain. These sublines occurred spontaneously, but similar mutants were induced with 10-fold increased frequency by treatment with mutagens, in which case they could be selected as clones in soft agarose. Scatchard plot analyses of ouabain bindin...
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I Edelen, D. G. B., "On the foundations of relativistic energy mechanics," Nuovo Cimento, 30, 292 (1963). 2 Einstein, A., "Hamiltonsches princip und allgemeine relativititstheorie," Sitzber. Pruessischen Akad. Wis&. (1916). 3 Thomas, T. Y., "On the geodesic hypothesis in the theory of gravitation," these PROCEEDINGS, 48, 1567 (1962). 4Taub, A. H., "General relativistic variational principle for...
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ژورنال
عنوان ژورنال: Somatic Cell Genetics
سال: 1979
ISSN: 0098-0366,1572-9931
DOI: 10.1007/bf01542643